动物实验 z-bo 2020-08-28 401

　　This study found that the combination of antibody precision and toxic oxygen may cause the loss of neuronal activity, resulting in the temporary loss of fear memory at the junction of mouse neurons (synaptic cognitive processes, such as chemical transmitters in () The transmission memory of (neurotransmitter) receptors is very important. By observing the phenomena after these receptors are inactivated, the function of the corresponding receptors can be understood. However, if the invalidity is accurate in location and time, this is only appearance information Although many technologies affect the cell surface and the internal structure of proteins by interfering with the action of receptors, neurotransmitter receptors usually only act on the cell surface. Some Japanese universities, such as Yokohama City University, Osaka University, and Tokyo University, A special collaboration was carried out on the induction method, which combines antibodies to produce a large amount of destructive oxygen (CALI: Chromophore Assisted Inactivation). Improved to inactivate proteins. This research was published in "Nature Biotechnology". Called

　　CALI technology is used to study protein function. Light radiation is used to generate a brief stream of toxic oxygen that can damage certain areas. The damage area is shorter than the traditional protein-protein interaction. In the current experiment, the researchers obtained an antibody other than the neurotransmitter receptor GluA1 and labeled it with a photosensitive molecule (photosensitizer). The antibody specifically inactivates the GluA1 receptor synaptic response in vitro and in mouse cells. Next, the research team injected the labeled antibodies into the hippocampus of the mouse brain to control memory and direction. Next, use the fear learning task to assess the impact on memory formation. The mice learned to walk between the cassette and the cassette, were affected by their feet in the cassette, and liked the light box. In previous studies, the research team used this task to deliver GluA1 to rat hippocampal synapses.

　　"We found that using green light to stimulate the hippocampus of mice made the mice return to the dark box faster than the control group." I pointed out the first author of the research group, Kato Takemoto. "This shows that GluA1 inactivated at the synapse cleared the fear memory in mice. When CALI was given within 2 hours after the completion of the first task, the GluA1 receptor was delivered to the synapse through bioelectric activity. This is the first A task was completed. They were unable to detect it after 24 hours. Researchers believe this is evidence that the GluA receptor has been replaced by other receptors that accept CALI, including GluA2 related proteins. With you

　　is consistent with the fact that you will not lose your fear memory within 24 hours