动物实验,雄激素缺乏 z-bo 2020-08-28 408

　　Objective: To investigate the effects of androgen deficiency on visceral fat deposition, serum hormones and inflammation-related gene expression in miniature pigs in a high-fat environment.

　　Method: The sexually mature male Wuzishan miniature pigs were randomly divided into three groups: castrate group (SHAM), castrate group (CAS), castrate plus testosterone group (CAS + T). The pigs in group 6 were fed a high-fat diet. At the end of the 12-week experiment, blood was drawn to detect changes in serum hormone levels, visceral fat was separated and weighed, and fluorescent quantitative PCR technology was used to detect genes related to fat formation, lipolysis, and inflammation. Expression detected.

　　Results: (1) Castration significantly reduced the serum testosterone content of high-fat piglets, but increased its serum leptin content. Exogenous testosterone treatment can restore the serum testosterone and leptin levels of castrated miniature pigs; (2) Adhere to a high-fat diet. Simple pig visceral fat accumulates through a high-fat diet. After testosterone treatment, the visceral fat content of castrated miniature pigs was significantly reduced. (3) Castration and testosterone treatment had no significant effect on the expression of fat metabolism genes FAS, ACC, HSL and ATGL. (4) Castration significantly increased the expression of inflammatory genes such as leptin, CD68, CCL16, CCL23 and SAA in the visceral fat tissue of high-fat mini-pigs, and the mini-pigs were cut with exogenous testosterone cast. I was treated. Pigs can reduce the expression of these genes.

　　Conclusion: Castration-induced androgen deficiency promotes visceral fat deposition in high-fat mini-pigs and up-regulates the expression of inflammatory genes in visceral adipose tissue. Treatment with testosterone can improve visceral fat accumulation and inflammation in castrated miniature pigs.