动物实验,神经干细胞 z-bo 2020-08-28 399

　　Objective: To clarify the neurobiological function of LSD1 by conditionally knocking out the LSD1 gene of mouse neural stem cells and observing the proliferation and behavior of mouse neural cells.

　　Method: Express LSD1 (flox/flox) transgenic mice, Nestin-cre (Tg) transgenic mice and Cre recombinase in neural stem cells, namely LSD1 (using Cre-LoxP system). Mating by breeding flox/flox). Estin-cre (Tg) genotype mice are LSD1 conditional knockout mice (LSD1-CKO) required for neural stem cells, and LSD1 (flox/flox) is used as a control mouse. Further immunofluorescence staining was used to detect neuronal proliferation in the DG area of the mouse hippocampus. The behavior of mice was also tested through experiments such as sugar water preference, forced swimming, and new object recognition.

　　Results: Compared with LSD1 (flox/flox) mice, the proliferation of hippocampal neurons in LSD1-CKO mice was significantly reduced (P = 0.023); the sugar water preference factor was reduced (P = 0.0075). In the forced swimming experiment, the immobility time increased significantly (P\u003c0.05); while the new object recognition experiment (P = 0.0019) showed a significant decrease in memory.

　　Conclusion: The mouse neural stem cells knock out the LSD1 gene, and the hippocampal neuron proliferation decreases. LSD1-CKO mice show negative mood and memory deficits.