动物实验,人羊膜间充质干细胞 z-bo 2020-08-28 459

　　Purpose: The study showed that human amniotic mesenchymal stem cells (human amniotic mesenchymal stem cells, hAMSC) have a variety of differentiation and proliferation functions, induce differentiation into liver-like cells, and thus have a certain repair effect on liver injury.

　　Methods: Human amniotic membrane mesenchymal stem cells were resuscitated and cultured in vitro. 66 healthy Wistar female rats were untreated and 22 were randomly selected as the normal control group. The remaining 44 rats were given white wine for 30 days to establish chronic alcoholic liver The injury model was randomly injected from the model group (1 mL PBS injected into the tail vein) and the hAMSCs group (1 mL hAMSCs (2x106)). Each component is 22. Four weeks after transplantation, serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), serum total bilirubin (TBIL) and serum albumin were used with an automatic biochemical analyzer. (ALB), total serum protein (Tp); simultaneously detect superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and catalase (CAT) in the liver of each group of rats And malondialdehyde (MDA), IL-4 content changes, immunofluorescence microscope observes the distribution of hAMSC labeled with PKH-26, TUNEL method detects hepatocyte apoptosis in each group

　　Results: Compared with the control group, the ALT, AST, TBIL of the model group were compared with the normal ALT, AST, TBIL of the hAMSCs transplantation group, and the model group with significantly increased Tp content was similar to the model group, and compared with the normal control. Compared with the model group, the Tp content was significantly lower (P\u003c0.05), and the ALB content of the model group was significantly lower than that of the model group. However, compared with the model group, the hAMSCs transplantation group had The content of ALB increased significantly (P\u003c0.05); the content of SOD, GSH-PX and CAT in the hAMSCs transplantation group increased significantly. In contrast, the contents of SOD, GSH-PX and CAT in the hAMSCs transplantation group were all decreased, while the contents of MDA and IL-4 were all increased (P\u003c0.05); the transplantation group was transplanted with PKH-despite 26 markers The positive hAMSCs cells were distributed in liver tissues, but no such cell distribution was found in other groups (P\u003c0.05); TUNEL method showed the apoptosis of hepatocytes in each group. The number of cells detected was the highest in the model group (34.27±5.71), followed by the hAMSC transplantation group (18.42±3.95). No apoptotic cells were found in the normal control group.

　　Conclusion: Human amniotic membrane mesenchymal stem cell transplantation can improve the blood biochemical indicators of liver cirrhosis in rats. hAMSC transplantation can reduce hepatocyte apoptosis in rats with chronic alcoholic liver injury.