动物实验 z-bo 2020-08-28 506

　　Objective: To investigate the process of acute kidney injury (AKI) in rabbits caused by a single intravenous injection of cisplatin.

　　Method: 40 New Zealand male rabbits were randomly divided into 5 groups, each with 8 rabbits. A single injection of cisplatin (5 mg/kg) through the ear vein was used to create an acute kidney injury model. According to the observation time, divided into cisplatin injection (T36) and 48-hour group (T48), 6-hour group (T6), 12-hour group (T12), 24-hour group (T24), 36-hour group (T48), rabbit intravenous The blood urea nitrogen (BUN) and blood creatinine (SCr) levels were measured at the corresponding time points before and after the injection. Use SPSS 22.0 statistical software for statistical analysis of the data. P\u003c0.05 showed that the difference was statistically significant. At each experimental node, the experimental rabbits were sacrificed, and kidney tissue specimens of each group of rabbits were obtained, and conventional paraffin sections were observed under an HE stained optical microscope.

　　Result: Compared with before injection, BUN and SCr levels of each group increased significantly after cisplatin injection. Twenty-four hours after modeling, all experimental rabbits in each group reached the standard of acute kidney injury, and the injury gradually deteriorated. Under the light microscope of HE staining, the changes in the T48 group were more obvious.

　　Conclusion: A single intravenous injection of cisplatin can cause acute kidney injury in rabbits, which gradually worsens within 48 hours.