动物实验,白血病 z-bo 2020-08-31 513

　　The research team led by Professor Ome Xie of South China Agricultural University studied the regulatory effects of miRNA on chicken leukemia, and gga-miR-375 as a tumor suppressor gene regulates the growth of cancer cells and plays an important role in the tumorigenesis of chicken leukemia.

　　J subgroup avian leukemia virus (JavianleukosisvirusJ, ALV-J) belongs to the retrovirus family, which causes chicken leukemia and various tumor diseases, and is widely spread in the poultry industry. To date, ALV-J infection in commercial and broiler chickens has caused significant economic losses to the global poultry industry. MicrornA (miRNA) is a small endogenous non-coding RNA that plays an important role in the occurrence and development of oncogenes and tumor suppressor genes. However, little is known about the potential role of miRNAs in chicken leukemia tumors. To this end, a research team led by Professor Ome Xie of South China Agricultural University has conducted research on miRNA regulation in chicken leukemia and published the results on PLoSONE. The researchers first detected the differential expression of miRNA between uninfected chickens and ALV-J-infected chickens through miRNA chips (a chip service provided by Lianchuan Biotechnology Co., Ltd.) and detected them in leukemia chickens. We found that gga-miR-375 is used, it is down-regulated and can target YAP1 oncogene to inhibit cell proliferation. Researchers found that overexpression of ga-miR-375 has a significant inhibitory effect on the proliferation of DF-1 cells, thereby significantly reducing the expression of yes-related protein 1 (YAP1).

　　Next, the researchers conducted an in vivo experiment, and the results showed that after 20 days of infection in chicken liver, gga-miR-375 was significantly down-regulated, while YAP1 was significantly up-regulated (P\u003c0.05). I found. The researchers also found that the expression of cyclin E, a key regulator of cell cycle progression, was significantly up-regulated during infection (P\u003c0.05). Drosophila apoptotic protein 1 inhibitor (DIAP1) is associated with caspase-dependent apoptosis and is also significantly up-regulated after infection. The research results show that gga-miR-375 as a tumor suppressor gene can regulate the growth of cancer cells and play an important role in the tumorigenesis of chicken leukemia.