动物实验,肾癌细胞 z-bo 2020-09-01 403

　　The research team of Dr. Miguel of China from the Institute of Biomedicine and Health of the Chinese Academy of Sciences discovered the cytotoxic mechanism of vitamin C on kidney cancer cells and its selective killing effect. For decades, people have been studying the role of vitamin C in cancer treatment. Clinical studies have shown that intravenous injection of high concentrations of vitamin C has a positive effect on cancer treatment, and in vitro experiments have shown that cancer cells are more likely to be vitamin C than normal cells. display. However, the mechanism of vitamin C cytotoxicity and this selective killing effect are still unclear.

　　The team used clear cell renal cell carcinoma (CCRCC) as a tumor cell research model. By studying the cytotoxic mechanism of vitamin C on kidney cancer cells, we found that vitamin C is mediated by hypoxia-inducible factor (HIF) cell death, which is also common in other cancer cells. possible. Using this as an entry point, HIF-positive renal cancer cells accumulate more intracellular vitamin C through the glucose receptor GLUT1, which is usually highly expressed by tumor cells and reacts with intracellular iron ions. Then it was discovered that more active oxygen could be produced.

　　OS causes severe DNA damage. Cell repairing DNA damage consumes a large amount of NAD + in the cytoplasm. HIF-positive kidney cancer cells rely on the ineffective glycolytic pathway to obtain energy through the "Warburg effect", so the ability to buffer the sudden drop of NAD+ is weak, so that it can irreversibly necrotic targets

　　This research is very important for the selective treatment of tumors and fills many gaps in this field.