动物实验,急性肾损伤,TRAP技术 z-bo 2020-09-01 431

　　Recently, USC stem cell scientists have successfully established a signal trap mouse (TRAPmouse) for acute kidney injury. With this TRAPmouse, you can catch the early signs of kidney failure. The explanation was published in the "Journal of Clinical Research". They use TRAP technology to generate new genetically modified mouse strains, extract cell and genetic information from various solid organs of mice, and use it to determine acute kidney injury.

　　TRAP technology was invented in 2008 by scientists at Rockefeller Medical School. TRAP technology can fluorescently label ribosomes of target cell types. Scientists can then collect the labeled ribosomes and determine which active genes "command" these ribosomes to produce protein. (TRAP stands for "Translation Ribosomal Affinity Purification".)

　　Based on this, the researcher Liu Jing of the University of Southern California and other researchers are more willing to capture mice with sick signals. We designed a simple and convenient method. A type of ribosome in which any of the thousands of existing genetically modified mouse strains can produce offspring when mated with disease-signaling mice. These offspring mice have specific organs or cell types with the offspring. mark. Liu and her colleagues used disease signal capture mice to label four different types of kidney cells and identified early signs of acute kidney injury. Surgery, infection or drug toxicity can cause 5% to 7% of hospitalized patients to suffer acute kidney injury, which can lead to chronic kidney disease or death.

　　Currently, doctors can test for acute kidney injury a full day after it has occurred. Mice that capture disease signals can detect disease signals early, which greatly increases the possibility of the patient's final diagnosis of the disease.