动物实验 z-bo 2020-09-02 335

　　Objective: To microinject K252a into the hippocampus to study its effect on rat behavior and classic depression-like indicators, and to establish a new depression model.

　　Method: SD rats were randomly divided into 5 groups: blank control group, sham operation group, chronic stress inhibition model group, intrahippocampal microinjection K252a group, intrahippocampus microinjection K252a combined with chronic stress group. It uses an opening experiment, a sugar water consumption experiment and a Morris water maze to determine the behavioral effects of rats. The ELISA method is used to detect changes in serum monoamine transmitter levels. Radioimmunoassay is used to observe the changes of plasma CRH, ACTH and CORT levels. Observe the changes of BDNF, CREB, ERK1/2 and BCL-2 protein expression in the hippocampus of western blotting rats.

　　Results: Compared with the blank control group, the activity level, sugar water consumption, learning ability and memory ability of the rats in the CUMS group were significantly reduced (P\u003c0.05 or P\u003c0.01), and HPA axis hyperfunction (P\u003c0) .01) and serum monoamines. The level of transmitter decreased (P\u003c0.01), and the expression of BDNF, CREB, ERK1/2 and BCL-2 was down-regulated (P\u003c0.01 or P\u003c0.05). There was no statistical difference in the above indicators in the DMSO group; DMSO, K252a and the comparison group, K252a + CUMS group activity, sugar water consumption, learning and memory ability were significantly reduced (P\u003c0.01 or P\u003c0.05), blood The level of manifest amine transmitter is reduced (P\u003c0.01 or P\u003c0.05), HPA axis function is significantly increased (P\u003c0.01 or P\u003c0.05), hippocampal BDNF, CREB, ERK1/2 and BCL The expression of -2 was significantly reduced (P\u003c0.01 or P\u003c0.05); compared with the CUMS group, there was no significant difference in rat indicators between the K252a group and the K252a + CUMS group; compared with the K252a group, the K252a + There was no significant difference in the above indicators in the CUMS group.

　　Conclusion: Through analysis from various perspectives of behavior, hematology and molecular biology, this model shows that the model is a classic chronic stress inhibition model in terms of surface validity, structural validity and functional validity. It has proved to be very similar. We can provide alternative research technology platforms for etiology research and antidepressant screening.