动物实验,PC12细胞凋亡 z-bo 2020-09-02 400

　　Objective: To investigate the protective effect of nimodipine on PC12 cell apoptosis induced by hydrogen peroxide (H2O2).

　　Method: The PC12 cells were randomly divided into normal group, model group (200μmol/LH2O2) and nimodipine low, medium and high concentration groups (1, 10, 100μmol/L nimodipine + 200μmol/LH2O2). The MTT method was used to determine the viability of each group of cells, Hoechst staining of apoptosis, aspartate proteolytic enzyme (caspase3), caspase-9 and superoxide dismutase (SOD) activities, and malondialdehyde (MDA) ) Colorimetric B lymphoma 2 (Bcl-2), Bcl-2 related X protein (Bax) and p53 protein content were detected, and Western blot analysis was performed.

　　Result: Compared with the normal group, the model group has reduced cell viability, increased apoptosis rate, increased caspase3 and caspase9, decreased SOD activity, decreased MDA content, decreased Bcl-2 expression, and decreased Bax. And showed that the expression of p53 increased. Everything is statistically significant (P\u003c0.01). Compared with the model group, low, medium and high concentrations of nimodipine increased cell viability, decreased cell apoptosis rate, decreased caspase 3 and caspase 9 activities, increased SOD activity, and increased MDA content. The expression of Bcl-2 protein increased, and the expression of Bax and p53 decreased. The difference was statistically significant (P\u003c0.01).

　　Conclusion: Nimodipine can inhibit the apoptosis of PC12 cells induced by H2O2 by regulating the expression of apoptosis-related proteins.