动物实验,细胞迁移 z-bo 2020-09-02 348

　　Objective: To construct HeLa and A549 cell lines that can knock down Smurf1 stably by lentivirus, and test the effect of knockdown on the migration of Smurf1 cells.

　　Method: Infect HeLa and A549 cells with packaged Smurf1 knockdown lentivirus, select puromycin-resistant positive cells after 7 days, Western blot and qPCR to detect knockdown effect, and execute Transwell to detect Smurf1 gene knockdown pair The impact of migration.

　　Result: The HeLa and A549 cell lines that stably knock down Smurf1 were successfully constructed using the interference lentivirus system, and the stable knockdown of Smurf1 inhibited the cell migration rate.

　　Conclusion: Knock down Smurf1 to inhibit cell migration.