动物模型,亚细胞 z-bo 2020-09-02 397

　　Micro two-photon fluorescence microscopy imaging technology has changed the way we observe cell and subcellular structures in freely moving animals

　　If you want to do something good, you first need to sharpen the tool. Better exploration of the human brain requires better tools and tools. Currently, one of the main directions of the National Brain Science Program is to create research tools for panoramic analysis of brain connection maps and functional dynamic maps. Among them, how to break scale barriers and combine micro-neural and synaptic activities with global brain activities and individual behavioral information is an important issue that needs to be resolved in this field.

　　This article mainly introduces the research results of "Ultra-high spatial-temporal resolution compact two-photon in vivo microscope system". In the past three years, Peking University Institute of Molecular Medicine, School of Information Science and Technology, Dynamic Imaging Center, School of Life Sciences, School of Engineering and Chinese Academy of Military Medical Sciences have successfully developed a new generation of fast, high-resolution small two-photon systems A multidisciplinary team was established. Fluorescence microscopes are used to obtain clear and stable images of brain neurons and synapses in freely moving mice. Compared with single-photon excitation, two-photon excitation has the advantages of superior optical tomography and deeper tissue penetration. The lateral resolution reaches 0.65μm, and the image quality is equivalent to that of a commercial large-scale desktop two-photon fluorescence microscope. A compact wide-angle microscope developed in the United States. The microscope can record real-time dynamic signals of dozens of neurons and thousands of synapses in the skull window of small animals. In large animals, it is also expected to achieve multi-probe attachment and long-term observation with multi-cranial windows in various areas of the brain.

　　In short, micro two-photon fluorescence microscopy imaging technology has changed the way we observe cell and subcellular structures in freely moving animals. It can be used for natural behavioral conditions such as foraging, breastfeeding, jumping, fighting, entertainment and sleep. During and after learning, long-term observation of multi-scale and multi-level dynamic changes in nerve synapses, neurons, neural networks, and remotely connected brain regions.