动物模型 z-bo 2020-09-02 381

　　Will drug use affect the health of future generations? Professor Ma Lan of the Institute of Brain Science of Fudan University and the National Institute of Medicine and Neurobiology said that the first and second generation offspring of cocaine addicted rats can also become cocaine addictive. This indicates that drug use may be transmitted to offspring.

　　A reporter from

　　Pengmei News (www.thepaper.cn) learned from the research team that the team used a rat cocaine self-management addiction model. This allows male mice to learn cocaine injection by touching the pedal when they need medicine. By continuously increasing the difficulty of the pedal task, the rats' desire for cocaine can be measured, which is the motivation for finding drugs. in

　　After

　　, the research team raised two groups of rats, the highest score (poisoned group) and the lowest score (poisoned group). Normal female rats were not exposed to cocaine to produce offspring and to observe whether it affects offspring. The test results show that the behavior of the offspring of the dependent group and the offspring of the non-dependent group is significantly different. Compared with non-addicts, first-generation offspring of addicts are more willing to trample to obtain cocaine.

　　Such high levels of poisoning can also be observed in the second generation offspring. This shows that the addictive behavior of cocaine is passed on from generation to generation.

　　More detailed research shows that the inheritance of addiction depends on whether the animal is actively seeking drugs, and whether the animal has a higher motivation to seek drugs and cocaine intake (for example, when the same as the rats in the addiction group When randomly selected addicted mice (dependency-like conditions) were injected with the same dose of cocaine, the addictive behavior was no difference between the group. At the same time, the experimental results of the research team showed that the addiction inheritance of cocaine to the offspring was caused by It is caused by positive drug discovery experience, not by mutations at the genome level. Therefore, it may be epigenetic or DNA-dependent. Genetic modification that can be inherited without changing the sequence. Addiction The comparison of the sequence and function of the DNA methylation modification of sperm DNA from non-poisoned rats reveals that the methylation changes of sperm DNA of addicted and non-poisoned rats are different, as well as some of the epigenetic changes. Maintained in the sperm DNA of offspring Transcriptome analysis also showed that the expression of neuroplasticity-related genes in drug-dependent brain regions has changed. These results indicate that the inheritance of addiction may be related to the epigenetic modification of sperm DNA and changes in the expression of brain-related genes.

　　This work was funded by the National 973 Program and the National Natural Science Foundation of China. Dr. Le Qiumin and Dr. Yan Biao are the co-lead authors of this article, and Professor Ma Lan is the corresponding author of this article.

　　Hama has been engaged in the research of drug addiction mechanism for many years. He is currently the dean of the Institute of Brain Science of Fudan University and the director of the Pharmacology Center of the School of Basic Medicine. She said that the study used experimental animal poisoning models, which showed that cocaine poisoning of parent rats would increase the risk of poisoning in offspring.