动物实验,心肌缺血 z-bo 2020-09-02 398

　　Objective: To observe the effect of Guanxinning Tablet (GXNT) on myocardial infarction and cardiac autonomic nerve function in rats with myocardial ischemia reperfusion injury (MI/RI).

　　Method: 70 SD rats were randomly divided into 7 groups, namely sham operation group, MI/RI group, GXNT 75mg/kg, 150mg/kg, 300mg/kg, 600mg/kg dose group. , And 300 mg/kg compound Danshen tablets (DST) group, 10 tablets in each group. All rats were given oral gavage for 7 days, and then the left anterior descending branch of the rats was ligated to establish an MI/RI rat model. During this period, the rat electrocardiogram was recorded, the J point of the electrocardiogram and the heart rate variability (HRV) index were analyzed, and Evans blue (EB) and 2,3,5-chlorotriphenyltetrazolium blue (TTC) were used to double the myocardium. dyeing. finished. HE staining was used to observe the myocardial infarct area and myocardial pathological changes to determine the plasma lactate dehydrogenase (LDH), creatine kinase (CK), malondialdehyde (MDA) and nitric oxide (NO) levels. done

　　Results: Compared with the MI/RI group, the Guanxinning tablet group and the compound Danshen tablet group significantly reduced the area of myocardial infarction and inhibited the increase of LDH and CK activity (P\u003c0.05, P\u003c0.01), During rat perfusion, the average value of ∑-J and J points (P\u003c0.05, P\u003c0.01), HRV and plasma NO levels increased significantly, and plasma MDA content decreased (P\u003c0.05, P\u003c0 .01) and improve the pathological state of myocardial tissue.

　　Conclusion: Guanxinning tablets can alleviate myocardial infarction in MI/RI rats and improve the autonomic nerve function of the heart.