动物实验,Fkbp51基因敲除 z-bo 2020-09-02 376

　　Objective: To study the effect of Fkbp51 knockout (KO) and wild-type (WT) mouse liver expression profiles on alternative splicing of genes in liver tissues.

　　Method: Use next-generation sequencing to sequence the liver expression profiles of Fkbp51 KO and WT mice, and use TopHat, KO and WT mice to perform alternative splicing analysis on RNA sequencing results to screen for differential retention of introns in liver tissue. , RI) and exon skipping (SE). Use the online tool DAVID to perform genetic (GO) and metabolic pathway (kyotoencyclopediaofgenesandgenomes, KEGG) enrichment analysis on these different alternative spliceosomes, and use the NCBI gene database to annotate these genes to do it.

　　Results: (1) The deletion of Fkbp51 may cause changes in the alternative splicing of mouse liver mRNA;

　　(2) Fkbp51 gene knockout may lead to changes in the expression of mouse liver mRNA alternative splicing;

　　(3) GO and KEGG analysis Through research, we found that these specially altered spliced genes are mainly related to the metabolism of fat-related derivatives, immunity and bile acid secretion.

　　(4) Genes related to differential intron retention are mainly related to the regulation of actin cytoskeleton and the metabolism of amino acids and their derivatives.

　　Conclusion: Fkbp51 gene knockout may change the alternative splicing of mRNA in the genome, thereby affecting the metabolic function of the mouse liver.