【Animal Experiment】-The effect of intrauterine injection of absolute ethanol on the estrous cycle of mice

  Objective: To investigate the effect of intrauterine injection of absolute ethanol on the estrus cycle of mice.

  Method: Using vaginal dissection cell staining method, 20 mice with regular estrus and intrauterine injection of absolute ethanol were selected. The estrus cycle was measured once a day during the first estrus cycle after surgery, and 10 mice were randomly selected for the second time. Measure the estrus cycle every 2 hours and observe the degree of change in the estrus cycle. I will.

  Result: After intrauterine injection of absolute ethanol, the overall estrus cycle of experimental mice was significantly prolonged, and the estrus and estrus period were disturbed to varying degrees.

  Conclusion: This experiment provides a concrete experimental basis for the clinical use of absolute ethanol by observing the disturbance of the estrus cycle of mice after the uterus is injured by absolute ethanol.