动物实验,鳞状细胞癌组织,口腔颊囊黏膜癌变 z-bo 2020-09-02 363

　　Objective: To investigate the expression and significance of EGFR, PDCD4, TGF-β1, Smad3 and Smad7 in normal oral mucosa, simple epithelial hyperplasia, epithelial dysplasia and squamous cell carcinoma tissues.

　　Methods: ELISA method was used to detect the serum EGFR and PDCD4 levels in the process of oral mucosal carcinogenesis in Chinese hamsters, and the normal TGF-β1 normal oral mucosal epithelium, simple hyperplasia epithelium, and abnormal hyperplasia were subjected to immunohistochemistry. Smad3 and Smad7 expressions were used to detect epithelium and scales Shape cell carcinoma tissue.

　　Result: During the carcinogenesis of oral mucosa, the serum EGFR protein showed an upward trend, and the serum PDCD4 expression showed a downward trend, showing a negative correlation. The expression of TGF-β1 and Smad7 in squamous cell carcinoma tissues was significantly higher than that of normal oral mucosa, simple epithelial hyperplasia and abnormal epithelial hyperplasia (P\u003c0.05), but the role of Smad3 is weak. Correlation analysis showed that oral dysplasia epithelium The expression of TGF-β1 in oral squamous cell carcinoma tissues was positively correlated with Smad7 (P\u003c0.05).

　　Conclusion: The expression of EGFR, PDCD4, TGF-β1, Smad3 and Smad7 are closely related to the biological behavior of oral squamous cell carcinoma. This experiment provides a theoretical basis for studying the mechanism of oral squamous cell carcinoma and affecting the development and prognosis of oral squamous cell carcinoma.