动物实验,免疫性脑脊髓炎 z-bo 2020-09-02 365

　　Objective: To study the significance of paclitaxel on the expression of CD28 and CTLA-4 in brain tissue of experimental autoimmune encephalomyelitis (EAE) rats and the content of B cell stimulating factor (BAFF) in the supernatant of spleen tissue.

　　Method: According to the random number method, 50 Wistar rats were divided into 5 groups: small, medium and high dose groups of paclitaxel (PTX) (PTX doses were 1 mg/kg, 2 mg/kg, 4 mg/kg). Normal control group, EAE control group, 10 animals in each group. Guinea pig spinal cord is used to produce GPSCH and mixed with the same amount of CFA to produce immune antigen. Inject into rat foot pads (2 mL/kg) for EAE modeling. After self-assembly, the PTX group was intraperitoneally injected with paclitaxel for 10 days. Both the normal and EAE control groups received 2 mL of 0.9% NaCl intraperitoneal injection. The brain tissue score was used to evaluate the inflammatory infiltration of the brain tissue of experimental rats. Collect rat brain tissue, and use flow cytometry to measure the content of CD28 and CTLA-4 in brain tissue. The spleen tissue was collected and used as a supernatant, and the spleen tissue was measured by ELISA. Medium BAFF content.

　　Result: The brain tissue score of each PTX dose group was lower than that of the EAE control group, and the difference between the groups was statistically significant (P\u003c0.01). The expression of CD28 in the brain tissue of rats in each PTX dose group was lower than that of the EAE control group. The difference between the groups was statistically significant (P\u003c0.01), the expression of CTLA-4 was higher than that of the EAE control group, and the difference between the groups was statistically significant (P\u003c0.01). The content of BAFF in the supernatant of spleen tissue was lower than that of the EAE control group, and the difference between the groups was statistically significant (P\u003c0.01).

　　Conclusion: PTX can reduce the neurological dysfunction score of EAE rats. Its mechanism is the expression of CD28 and CTLA-4 in rat brain tissue and the content of BAFF in the supernatant of spleen tissue. It has a preventive effect on EAE because it includes EAE adjustment.