动物实验,环磷酰胺 z-bo 2020-09-03 431

　　Objective: To explore the effects of different routes and doses of cyclophosphamide on embryos and fetuses of pregnant rabbits, and to determine the best administration method for induction of fetal rabbits.

　　Method: pregnant rabbit normal saline control group C, intravenous injection Y1 group (15mg/kg), subcutaneous injection low-dose Y2 group (20mg/kg), subcutaneous injection high-dose Y3 group (30mg/kg) (kg)), and follow The corresponding route of GD10-13 (GD0 on the conception day) was administered at all doses in each group. GD28 anatomical structure, number of corpus luteum, number of implants, continuous uterine weight, absorption rate confirmation, fetal collection, fetal gender, length, tail length, live birth, stillbirth confirmation and necessary inspection based on external deformation, internal deformation, bone inspection and deformation .

　　Result: The pregnant rabbit fetus has malformations in the intravenous and subcutaneous low-dose cyclophosphamide groups. The appearance deformities of the two groups were 30.77% and 95.65%, skeletal deformities were 7.69% and 73.91%, and visceral deformities were respectively. The ratios are 20.51% and 47.83% respectively. The incidence of fetal absorption in the high-dose subcutaneous injection group was 100%.

　　Conclusion: Subcutaneous injection of 20 mg/kg cyclophosphamide and GD10-13 in pregnant rabbits can be used as a positive dose of rabbit embryo embryo toxicity test. This method has a short administration cycle, easy operation, low fetal absorption rate, and many fetal malformations. It can provide a basis for colleagues to choose a suitable rabbit embryo-fetal developmental toxicity model.