动物实验,大肠杆菌 z-bo 2020-09-03 406

　　Objective: Prokaryotic expression and purification of Asprosin protein in Escherichia coli, and study its effect on improving cardiac function.

　　Method: Obtain the asprosin coding sequence from GenBank, optimize the codon according to the E. coli codon preference, synthesize the entire gene, connect it to the expression vector, and perform IPTG-induced expression and purification. The mouse heart dysfunction model was established by ligating and relaxing the left anterior descending branch. Thirty mice were randomly divided into 3 groups: sham operation group (sham operation), cardiac dysfunction group (MI/R) and cardiac dysfunction group (MI/R +Asp). Echocardiography examines left ventricular function, assesses the degree of cardiac dysfunction, and studies the effect of asprosan on improvement of cardiac function.

　　Result: After prokaryotic expression and purification, the purity of the target protein is more than 95%, and the endotoxin content is less than 0.1EU/μg protein, which is suitable for cell and animal research. Successfully established a cardiac dysfunction model. Compared with the simple injury group, after exogenous administration of recombinant asprosin protein, the cardiac function of the mice was significantly improved (P\u003c0.05).

　　Conclusion: Asprosin protein can inhibit the damage of heart function and improve heart function.