动物实验,蛋白激酶 z-bo 2020-09-03 380

　　Objective: To investigate the effect of glucose supplementation on AMPK activation of exercise myocardium by measuring changes in AMP-activated protein kinase (AMPK) activity and glycogen content in rats after acute exercise.

　　Method: Rats perform acute endurance exercise and supplemented with different doses of supplementary glucose at different times before and after exercise. Western blotting was used to measure the dynamic changes of AMPK activity in the rat heart, while antron was used to measure myocardial glycogen content.

　　Result: Exercise caused a significant increase in AMPK activity in the rat's myocardium, and maintained a high level 1 hour after acute exercise, while it did not increase significantly in the supplementary exercise rat's myocardium. Neither exercise nor low-dose sugar supplementation caused significant changes in the glycogen content of rats.

　　Conclusion: (1) Acute exercise can increase the activity of AMPK in the rat myocardium, and glucose supplementation can significantly inhibit the activation of AMPK during and after exercise. (2) High-dose sugar supplementation after exercise can effectively increase myocardial glycogen content 24 hours after exercise.