动物实验,糖尿病 z-bo 2020-09-03 377

　　Objective: To investigate the effect of aerobic exercise on pancreatic islets and β cells in type 2 diabetic rats and its mechanism.

　　Method: 30 Wistar rats and 10 rats were randomly selected as group C, and the remaining 20 rats were given streptozotocin (STZ) and a high-fat and high-sugar diet to establish a type 2 diabetes rat model. The 15 successful animals were divided into two groups, one group was in the DMC group (n=7), and the other group was in the DME group (n=7, 1 animal died at the end of the experiment). The rats in the DME group exercised on a treadmill at an intensity of 20 m/min per day for 30 minutes, and rested for 1 day every 6 days for a total of 8 weeks. The rest of the rats were free to move in the cage. At the end of the experiment, blood was drawn from the abdominal aorta to measure blood glucose and serum Ins content and calculate HOMA. The first third of the pancreas was cut into paraffin sections, HE stained, and the islet morphology and β cell structure were observed digitally and photographed. In the magnification field, use MoticImagesAdvanced3.1 image processing software to measure the perimeter and area of the island, and calculate the SF accordingly. After homogenizing the rest of the pancreas, measure its Ins content, GK and track total ATPase activity.

　　Result: The islet circumference and area of the DME group were significantly increased than that of the DMC group (P\u003c0.05), but lower than that of the C group, and the SF was significantly increased. done. Compared with the rats in the DMC group, β-cell hypertrophy, vacuoles and nuclear coagulation and contraction were significantly improved. SF and HOMA are also significantly higher (P\u003c0.05).

　　Conclusion: Aerobic exercise reduces the blood glucose level of type 2 diabetic rats by increasing GK, ATP synthase activity and insulin sensitivity in pancreatic tissue, and improves the morphology and structure of pancreatic islets and pancreatic β cells.