动物实验,2型糖尿病 z-bo 2020-09-03 413

　　OBJECTIVE: To compare the ulcer healing characteristics of C57BL/6J mice induced by C57BL/6J-db/db and STZ with the natural mutations of commonly used genes in type 2 diabetic mice, and to establish a stable diabetic mouse ulcer model to provide evidence for related animal studies .

　　Method: Establish a diabetic mouse ulcer model and calculate the dynamic healing rate and healing time of mouse wounds at 0, 3, 5, 7, 10, and 14 days. Histology and Masson staining, immunohistochemistry (CD31 and PCNA) to observe the pathological changes of the wound at 7 and 14 days; fluorescence determination of the gene expression of the healing-related factors collagen-α and α-SMA determines the result: gene mutation db/db The wound healing time of mice was significantly delayed from (16.6±0.8) d to (20.2±1.3) d (P\u003c0.001); compared with STZ-induced mice, the db/db group was compared with STZ. The granulation tissue in the group grew slowly, the length of new epithelium was insufficient, the deposition of collagen was blocked, and the wound healing was not good; CD31 and PCNA in the db/db group were significantly under-expressed on day 7 (P\u003c0.01); on day 7 and 14 On days, genes in the db/db group were up-regulated, and its multiple was significantly lower than that in the STZ group.

　　Conclusion: Both diabetic mice delayed wound healing, but compared with STZ-induced mice, genetically mutant mice are more suitable for diabetic ulcer wounds in terms of delayed wound healing and difficulty in healing.