【Animal Experiment】-The influence of air sac on the results of abdominal wall withdrawal reflex experiment

  Purpose: To select suitable materials to prepare balloons for abdominal wall retraction reflex experiments.

  Method: Use digital display calipers and X-ray images to measure different airbag diameters at different pressures, and use the 3-point pain threshold as a guideline to test whether the pressure value of the rat is caused by obvious visceral pain.

  Result: The diameter of the round latex balloon changes uniformly and slowly as the pressure value increases, filling the pressure range commonly used in experiments (20-80mmHg). The threshold of causing visceral pain in rats is close to the harmful stimulus value. Cause irreversible damage to the intestines of rats.

  Conclusion: Round latex balloons are very suitable for abdominal contraction reflex experiments.