动物实验,Mac-1基因,黑色素瘤 z-bo 2020-09-04 458

　　Objective: To investigate the effect of Mac-1 loss on the growth of melanoma.

　　Method: Expand Mac-1 knockout (Mac-1-/-) mice to get as many mice as needed for the experiment. A control group of C57BL/6J mice and an experimental group of Mac-1-/- mice were established, and B16-F10 cells were injected subcutaneously. Calculate the viability of mice, measure the volume and weight of tumors, and detect tumor cells and tumor cell proliferation by immunohistochemical staining. Macrophage infiltration.

　　Result: The survival rate of melanoma mice after Mac-1 deletion is significantly different from that of C57BL/6J mice in the control group (P\u003c0.001), and the tumor volume and weight in the control group are C57BL. This is significantly different from the survival rate of 6J mice. (P \u003cu\→ u003c0.001), the number of proliferating cells in the tumor tissue is significantly different from that in the control group (P'u003c0.01), and the infiltration of macrophages in the tumor tissue is significantly reduced.

　　Conclusion: The deletion of the Mac-1 gene significantly inhibited the growth of melanoma.