动物实验,SIRT1基因,骨关节炎 z-bo 2020-09-04 465

　　Objective: To establish a related mouse osteoarthritis model by knocking out the SIRT1 gene, and to observe the morphological differences of cartilage tissue sections through various separate staining or composite staining techniques.

　　Method: The mouse knee joint tissue samples were divided into two groups: SIRT1-mouse sham operation group (n = 6, group A), SIRT1-mouse osteoarthritis model group (n = 6, group B). The knee joint osteoarthritis model was established by anterior cruciate ligament transection and medial meniscus resection. HE staining, Safranin O Fast Green Stain, Safranin O Alcian Blue Stain, Safranin O Stain, Fast Green Stain, Neoblue Stain, used to observe the morphological changes of Alli knee cartilage, 6 separate stains Agent or combination dye method.

　　Result: The saffron O-fast green and saffron O-Alcian blue staining were used to observe the cell morphology of chondrocytes, the layered structure of cartilage, the display of type II collagen fibers, the changes of tide lines and subchondral bone. Among them, the effect is better; Safranin O staining and Alcian blue staining have certain advantages in observing cartilage tissue loss.

　　Conclusion: When observing the histological sections of knee osteoarthritis in SIRT1 knockout mice, compared with single staining, composite staining has more obvious advantages in obtaining various information about cartilage structure.