动物实验,小胶质细胞炎症 z-bo 2020-09-04 382

　　OBJECTIVE: To observe the influence of sodium metavanadate (NaVO3? 2H2O) exposure on the inflammatory response and migration of microglia, and to explore the related mechanisms of vanadium neurotoxicity.

　　Methods: The primary cultured SD rat microglia were incubated with sodium metavanadate. Immunofluorescence technology showed the morphological changes of microglia and the expression of specific marker Iba1. Western blot technology was used for iNOS and COX-2, . Enzyme-linked immunosorbent assay was used to detect the expression of ERK and p-ERK protein, the release levels of inflammatory factors TNF-α and IL-1β, the construction of a scratch migration model, and the immunofluorescence to record the migration of sodium metavanadate on microglia Technical impact.

　　Results: After incubating microglia with sodium metavanadate, the morphology of microglia changed from resting state to phagocytic-like, and the expression of specific markers Iba1, iNOS and COX- was significantly increased compared with the two control groups , TNF-α and IL-1β protein expression increased significantly, and sodium metavanadate promoted the migration of microglia.

　　Conclusion: Sodium metavanadate significantly promotes the inflammatory response and migration of microglia.