动物实验,miR-424 z-bo 2020-09-04 416

　　Objective: To investigate the effect of miR-424 on the migration and invasion of non-small cell lung cancer A549 cells.

　　Methods: Detection of miR-424 and lipid expression in lung cancer cells NCI-H460, NCI-H1975, NCI-H446, A549, NCI-H1299, NCI-H157 and human embryonic lung fibroblasts MRC-5 RT-PCR method Lipofectamine ?2000 transfer miR-424 inhibitor and miR-424NC to A549 cells. 48 hours later, the expression of miR-424 was detected by RT-PCR method, cell viability was detected by CCK-8 method, cell migration ability detected by scratch test, cell invasion ability detected by Transwell test, matrix metal detected by Western blot Expression of protease 2 (MMP2), MMP9, transforming growth factor β1 (TGF-β1) and p-Smad3.

　　Results: miR-424CI-H460, NCI-H1975, NCI-H446, A549, NCI-H1299, NCI-H157 ((1.78±0.13), (1.69±0.10), (1.89±0.18)) in lung cancer cells, ( 2.88±0.27), (2.52±0.20), (2.49±0.23)] miR-424 expression level is (0.58±0.05) miR-424 expression level in human embryonic lung fibroblast MRC-5. Significantly higher than this value (P \u003c0.01). Compared with the miR-424NC group, the miR-424 expression in the miR-424 inhibitor group was significantly reduced (P\u003c0.01), cell viability was reduced (P\u003c0.01), and cell migration and invasion capabilities were reduced (P≥ 0.01), and the expression levels of MMP2, MMP9, TGF-β1 and p-Smad3 were significantly down-regulated (P≥0.01).

　　Conclusion: The down-regulation of miR-424 can inhibit the migration and invasion of A549 cells by inhibiting the TGF-β1/Smad3 signaling pathway.