动物实验,心肌梗死 z-bo 2020-09-04 383

　　Objective: To investigate the effect of simvastatin local ossicle injection on angiogenesis and heart function after myocardial infarction in rats.

　　Method: Wistar rats were randomly divided into sham operation group, myocardial infarction model group and intraosseous simvastatin group (n = 12). Ligation of the left anterior descending coronary artery to establish a rat model of myocardial infarction. 24 hours later, the experimental group injected 0.5 mg of simvastatin into the left tibia. Four weeks later, the left ventricular function of the small animal was evaluated by echocardiography. The area of myocardial infarction was calculated by triphenyltetrazolium chloride (TTC) staining and immunofluorescence staining to detect local angiogenesis.

　　Results: The echocardiographic results showed that the left ventricular systolic function was significantly reduced after 4 weeks of myocardial infarction, and intraosseous injection of simvastatin did not significantly improve the left ventricular function after myocardial infarction in rats. TTC staining showed no significant reduction in the area of myocardial infarction in the group of intraosseous injection of simvastatin. Immunofluorescence staining showed that the myocardial blood vessel density of the simvastatin intraosseous group did not increase significantly.

　　Conclusion: A single injection of a small dose of simvastatin (0.5 mg) into the bones of rats 24 hours after myocardial infarction does not significantly improve the areas of myocardial infarction, angiogenesis and heart function.