动物实验,环磷酰胺 z-bo 2020-09-04 404

　　Objective: To study the changes of metabolic pathways during the immunosuppressive effect of cyclophosphamide on mice by metabolomics methods.

　　Method: Use flow cytometry to understand the changes of immune cells, and establish and judge multiple results of partial least squares models. With the help of metabolomics small molecule compounds, software for identifying metabolites, screening of variable potential biomarkers with the importance of some least squares model projection (VIP) parameters, and rapid identification and analysis of identified metabolites through statistical analysis For further identification and analysis, the system finally identified 9 different metabolites through database search and calculation, and analyzed 3 different metabolic pathways through the results of pathway enrichment.

　　Result: Cyclophosphamide regulates all metabolic pathways of normal human unsaturated fatty acid biosynthesis, mitochondrial fatty acid metabolism, glycerophospholipid metabolism, steroid hormone biosynthesis, arachidonic acid metabolism, fatty acid metabolism and pyrimidine biosynthesis. I'm waiting. It has obvious effects.

　　Conclusion: The most important metabolic pathways affected by cyclophosphamide in the normal and experimental groups are arachidonic acid metabolism, glycerophospholipid metabolism and pyrimidine metabolism.