动物实验,姜黄素对N-甲基亚硝基脲 z-bo 2020-09-04 425

　　Objective: To analyze the chemical intervention and mechanism of curcumin on N-methylnitrosourea (MNU) induced bladder cancer rat model.

　　Method: 100 SD rats were randomly divided into 4 groups, namely control group (10), model group (10), intervention group (40), treatment group (40), control group, isochronous bladder I injected saline. The other three groups were injected with MNU into the bladder to induce the formation of SD rat bladder cancer model (1 mg/mL MNU solution was injected into the bladder, the time of MNU injection was 2nd, 4th, 6th and 8th weeks, 2 times each time mg, once every 2 weeks, 4 times in total), when bladder cancer is induced in rats, distilled water is infused into the model group and MNU is infused into the bladder. If the intervention group was perfused with curcumin solution (400μmol/L), the bladder was perfused at 1, 3, 5, 7, and 9 weeks, and the rats were euthanized at the 10th week. In the treatment group, after inducing the rat bladder cancer model, the bladder was perfused with curcumin solution (400μmol/L). Bladder perfusion lasted 14, 16, and 18 weeks. The rats were sacrificed at the 19th week. The obtained bladder tissue was stained with hematoxylin-eosin (HE) to observe pathological changes. TUNEL end labeling method Apoptosis was used to identify tumor tissues; Western blot was used to detect the expression of apoptosis-related proteins.

　　Results: The incidence of bladder cancer in the model group was 90% (9/10) at 10 weeks, and the incidence of rat bladder cancer in the intervention group was 12.5% (5/40) at 10 weeks. .. The incidence of bladder cancer in the treatment group at week 10 was 92.5% (37/40). There is a significant difference in the incidence of bladder cancer between the intervention group and the model group (P≥0.05), indicating that curcumin has a greater effect on MUN-induced bladder cancer. Rats have obvious chemical intervention effects. Curcumin was administered to the treatment group after the formation of bladder cancer. The incidence of bladder cancer at week 19 was 78.4% (30/37). Compared with the 10 weeks before treatment, curcumin has been shown to affect bladder cancer. It is therapeutic and can slow the progression of bladder cancer. TUNEL experiment confirmed that curcumin can significantly promote the apoptosis of bladder cancer cells and inhibit the proliferation of bladder cancer cells. The results of Western blot showed that curcumin inhibited the activation of NF-κB and effectively down-regulated the expression of NF-κB-regulated gene products.

　　Conclusion: Curcumin has an important chemical intervention effect in MNU-induced bladder cancer rat model. Its mechanism of action inhibits the activation of NF-κB and effectively down-regulates the gene products regulated by NF-κB. It can inhibit proliferation and induce cell apoptosis. Carry out anti-cancer chemical interventions and prevent bladder cancer recurrence, and express related proteins in the culture medium to regulate bladder cancer.