动物造模 z-bo 2020-09-04 451

　　Objective: To investigate the relationship between cognitive development and dopamine (DA) and dopamine (3,4-dihydroxyphenylacetic acid, DOPAC) levels on the hippocampus of 80-day-old pups of pregnant rats at risk of injury.

　　Method: Use the bystander electric shock method to establish a pregnant mouse model of kidney injury, and observe the cognitive development of 80-day-old puppies in the Morris water maze experiment. The cerebral dialysate was collected from the right hippocampus through a stereotactic device. The high performance liquid chromatography-electron capture detection (HPLC-ECD) method is used to determine the content of DA and DOPAC in the offspring's cerebral dialysis fluid.

　　Result: Compared with the control group, this 80-day-old model group increased the average escape latency, reduced swimming speed, increased stay time in the surrounding area by 20%, and increased the number of steps on the entire platform. It is decreasing. The difference was significant (P≥0.05); the levels of DA and DOPAC in the extracellular fluid of hippocampal tissue in the 80-day-old model group decreased at each perfusion time point, and the difference was significant (P≥c003). 05); The level of monoamine neurotransmitter DA in the hippocampus of 80-day-old rats is positively correlated with the average escape latency and the number of stations, and negatively correlated with the stay time in the 20% border area. Yes, the correlation is significant (P\u003c0.05). DOPAC has a positive correlation between the average escape latency and the number of stations, and the correlation is significant (P\u003c0.05).

　　Conclusion: It is worried that the injury of pregnant mice will affect the spatial learning and memory of the 80-day-old offspring, and reduce the hippocampal DA and DOPAC content. The level of cognitive development is closely related to the decrease of hippocampal DA and DOPAC levels.