动物实验,褐色脂肪组织 z-bo 2020-09-05 377

　　Objective: To explore the effect of low temperature and low pressure environment on brown adipose tissue in mice.

　　Method: Twenty-four 6-week-old adult male C57BL/6N mice were selected, and their individual health conditions were uniform and divided into 4 groups, each with 6 mice. Their normal temperature and pressure (18-22°C, altitude 20-60m) ) ). ), normal temperature/low pressure (18-22°C, altitude 5000m), normal pressure/low temperature (0-6°C, altitude 20-60m), low temperature/low pressure (0-6°C, altitude 5000m). The experiment time was 4 weeks. Body weight was measured at the beginning and the end of the experiment. After the experiment, the back and groin fat were completely removed, and the back and groin fat tissues were stained with HE. The brown fat marker UCP-1 is used to measure qPCR molecular expression and Western blot protein expression.

　　Results: In the cold, low pressure, cold and normal pressure, and room temperature and low pressure groups, the weight gain of mice was significantly lower than that of normal control mice in the same period; the back of cold, cold and normal pressure mice. The inguinal adipose tissue is darker and the blood supply is abundant. The adipose tissue morphology of the normal temperature and antihypertensive group is larger than that of the other groups. The staining results show that the back of the mouse has more fat cells. The cells are small, dark, and are typical of brown fat cells in morphology; UCP- is a brown fat marker in the back fat tissue of mice under cold conditions. Under low pressure conditions, 1 is highly expressed at mRNA and protein levels, and Only up-regulated at the mRNA level.

　　Conclusion: The low temperature and low pressure conditions that simulate the plateau environment have a stimulating effect on the formation of brown adipose tissue in mice, and this effect is related to low temperature.