动物模型,二甲双胍 z-bo 2020-09-05 353

　　Objective: To study the effect of metformin supplementation on the learning and memory ability of mice during the aging process caused by D-galactose and its possible mechanism.

　　Method: 24 ICR female mice were randomly divided into 3 groups: control group, aging group, and aging metformin group. Each group received 8 mice continuously for 16 weeks. Monitor the body weight and food intake of each group of mice. Behavior tests the learning and memory abilities of mice. The histology of mouse hippocampus was observed by HE staining. Colorimetric method was used to detect the hippocampal glutathione (GSH) level of each group of mice.

　　Results: Compared with the aging group, the mice in the aging metformin group lost weight (P\u003c0.05), and the escape latency and swimming distance of Morris water maze were significantly reduced (P\u003c0.01 or P\u003c0.05) and prolonged The swimming time of the target quadrant (P\u003c0.05), the swimming speed is increased (P\u003c0.05); the number of active avoidance in the space shuttle experiment is increased (P\u003c0.05); HE staining shrinks the hippocampal dentate gyrus , And significantly reduced the deeply stained hippocampal neurons. The level of glutathione in the tissue increased significantly (P\u003c0.05).

　　Conclusion: Supplementing with metformin can significantly slow down the decline of learning and memory in the aging process of mice, and maintain the normal structure of hippocampal neurons. This mechanism may be related to the weight loss of mice and the increase of hippocampal antioxidant levels.