动物造模,脓毒症小肠损伤 z-bo 2020-09-05 406

　　Objective: To investigate the effect of oat β-glucan on the expression of small intestinal injury-related genes in sepsis.

　　Method: SD rats were randomly divided into normal control group (NC group), experimental control group (TC group) and oat β-glucan group (Oglu group). The sepsis model was established in the TC and Oglu groups by cecal ligation and puncture (CLP). The Oglu group received different doses of oat β-glucan by gavage. After 12 hours, the small intestine tissue was collected from each group, and Western blot was used to detect the expression of damaged genes in the small intestine tissue.

　　Results: 1) Oat β-glucan reduced the small intestinal damage caused by sepsis in the small intestine tissue of septic rats, and reduced the levels of TNF-α, IL-1β, and IL-6 (P\u003c0. 05); (2)) In the sepsis group, the expression of small intestinal injury-related protein Bcl-2 was down-regulated, and the expression of Bax, caspase-3, and Toll-like apoptosis genes FAS, FASL and NF-κB increased significantly. (3) The expression of small intestinal mucosa Bax, caspase 3, Toll-like apoptotic genes FAS, FASL and NF-κB in oat β-glucan group was lower than that in sepsis group, while the expression of Bcl-2 was higher.

　　Conclusion: Oat β-glucan regulates the expression of small intestinal injury genes in sepsis rats and protects the small intestinal epithelium from sepsis.