动物造模,肠黏膜损伤,姜黄素 z-bo 2022-10-07 410

　　Objective: To investigate the protective effect of curcumin pretreatment on the intestinal mucosa of rats in desert, dry and hot environments.

　　Method: 80 male SPF rats were randomly divided into a normal saline control group (NC group) 40 rats and curcumin pretreatment group (HDC group) 40 rats. The C group was given normal saline in the stomach, and the HDC group was given 200 mg/kg curcumin once a day for 7 days. The rats in each group were placed in a laboratory that simulated the dry and hot environment in the desert. Temperature: (41±0.5)°C, relative humidity: (10±2)%. At 0, 50, 100 and 150 minutes, rats in the NC group and HDC group (10 rats in each group) were randomly selected and injected intraperitoneally with sodium pentobarbital for anesthesia and pathological observations were performed. Intestinal samples were taken due to oxidative stress. Excitation indicator CAT, SOD, MDA detection.

　　Result: At 0 and 50 minutes, the pathological damage score of HDC group was not significantly different from that of NC group (P\→0.05). At 100 and 150 minutes, the pathological damage score of the HDC group was significantly lower than that of the NC group (P \u003cu003c0.01). At 50, 100 and 150 minutes, the CAT and SOD activities of the HDC group were significantly higher than those of the NC group (P\u003c0.05 or P\u003c0.01), and the MDA content of the HDC group was significantly higher than that of the NC group. Lower (P\u003c0.05 or P\u003c0.01). Exposure to dry heat, the intestinal mucosal pathological damage score of the NC group was negatively correlated with CAT and SOD activity (r = -0.9128, r = -0.9125, both are P\u003c0.01), and MDA was positively correlated with content (r = 0.9258 , P\u003c0.01).

　　Conclusion: Curcumin pretreatment has a protective effect on rat intestinal mucosa in the desert dry and hot environment, and its mechanism may be related to the inhibition of curcumin intestinal oxidative stress.