动物造模,OPG-RANK-RANKL z-bo 2020-09-05 382

　　Purpose: Use the osteoprotegerin-nuclear factor kappa B receptor activator (OPG-RANK-RANKL) system to carry out different dietary metabolism in developing WHBE rabbits. Study the effects of natural calcium levels.

　　Method: 21 weaned WHBE rabbits were randomly divided into 3 groups (group I, II, and III). The calcium levels were 0.95%, 1.10%, 1.30% and other basically the same nutritional levels. I invite you to eat. The test period is 42 day. At the end of the experiment, the rabbit serum calcium (Ca), parathyroid hormone (PTH) and bone-derived alkaline enzyme (BALP) levels were measured. OPG-RANK was performed on WHBE rabbit bone tissue using fluorescence quantitative PCR and immunohistochemistry. -RANKL gene transcription and protein expression analysis. Finally, the RANKL/OPG ratio was used as an index to evaluate the correlation between WHBE rabbit bone metabolism and dietary calcium levels.

　　Results: The experimental results showed that there was no significant difference in serum Ca, PTH content and BALP activity in groups I, II, and III (P\→0.05). The expression level of RANKL mRNA and the ratio of RANKL/OPG mRNA in WHBE rabbit bone tissue were the lowest in the second group, which was significantly different from the first and second groups (P\u003c0.05). Calcium level has an important influence on OPG-RANK-RANKL protein expression in WHBE rabbit bone tissue (P\u003c0.01). The positive index of OPG protein expression in group II and O was significantly higher than that in group I (P\u003c0.01), while the positive index of RANK protein expression in rabbit bone tissue in group II. Significantly lower than the first and third groups (P\u003c0.01). In group II, the ratio of RANKL/OPG protein expression was the lowest, which was significantly different from group I (P\u003c0.01). In addition, the ratio of RANKL/OPG mRNA in the bone tissue of WHBE rabbits and the positive index ratio of protein expression showed a significant secondary correlation with the calcium content in the diet (R2 = 0.4068, 0.8433, P\u003c0.05). , P\u003c0.001).

　　Conclusion: There is a significant correlation between the bone metabolism of the growing WHBE rabbit and the calcium level in the diet. When the calcium content in the diet is 1.10%, the growing WHBE rabbit can reach the ideal bone metabolism level.