动物造模,组胺对抑郁症 z-bo 2020-09-05 384

　　Purpose: Do you want to study the effect of histamine on the neurons in the hippocampal C1 area of SD rats and the behavior of depressed rats?

　　Method: Observe the direct effect of histamine on the firing rate of neurons in the hippocampus C1 area in vitro by extracellular recording of isolated brain slices? How to record the electrical stimulation of somatic cells and the hypothalamus, and observe the effect of endogenous histamine on the firing frequency of hippocampal neurons? How to observe the changes in motor and spatial memory requirements in the field and Morris water maze experiment in model rats after depression, and microinjection of histamine into the hippocampal C1 area?

　　Result: The extracellular records of the isolated brain slices show that histamine affects hippocampal nerves through H1 receptors. It has a concentration-dependent excitatory effect; extrasomatic recordings indicate that endogenous histamine exhibits a bidirectional effect of short-term excitement and long-term inhibition on hippocampal neurons. In behavioral experiments, injecting histamine into hippocampal C1 area caused depression, while in field experiments, rats' appetite for exercise was significantly reduced. In the Morris water maze experiment, the spatial memory ability was significantly reduced. However, can injection of histamine H3 receptor blocker clobenProPit significantly improve the desire for movement and spatial memory in depressed rats?

　　Conclusion: Histamine has a two-way effect on neurons in the hippocampal C1 area. It has different degrees of initial excitation and subsequent inhibition at the cellular and global levels in vitro and in vivo. It has a comprehensive inhibitory effect and can exercise depressed rats. Can it weaken desire and spatial memory, and the histamine H3 receptor blocker clobenProPit can improve the movement and spatial memory cravings of depressed rats?