动物造模 z-bo 2023-01-12 485

　　Objective: To compare the effects of different intervention temperatures on endoplasmic reticulum stress and pressure ulcer apoptosis in rats, and to provide experimental evidence for clinical prevention and treatment of pressure ulcers.

　　Method: 40 SPF adult male SD rats with pressure ulcer model established by ischemia-reperfusion were randomly divided into 4 groups: sham operation group (sham operation group), model group, heat intervention group, and cold war intervention group. At the end of the experiment, the muscles of each group of compressed parts were incised on ice. Use HE staining to observe the pathological changes of skeletal muscle; Western blot shows that the endoplasmic reticulum stress-related protein GRP78? Whether to detect the expression level of casPase-12 and CHOP; observe the expression of casPase-12 and CHOP by immunofluorescence; observe cell apoptosis by TUNEL staining?

　　Result: Compared with the model group, skeletal muscle cell damage and apoptotic cells increased in the heat intervention group. From the results of immunofluorescence, caspase? From the results of western blotting, the positive expression of GRP78-12 and CHOP increased. The expression of casPase-12 CHOP and CHOP was higher than that of the model group (P≥0.05), while the degree of skeletal muscle cell damage and apoptotic cells were reduced in the cold intervention group. Immunofluorescence results showed that the positive expression of caspase-12 and CHOP decreased. Western blotting results showed that GRP78? Are both casPase-12 and CHOP equations lower than the model group (P\u003c0.05)?

　　Conclusion: Local cold intervention can reduce the damage of pressure ulcers in rats by inhibiting the apoptotic pathway caused by endoplasmic reticulum stress. Local thermal intervention will intensify. Will ERS accelerate the development of rat cell apoptosis and exacerbate pressure ulcer injury?