动物造模 z-bo 2020-09-05 371

　　OBJECTIVE: To establish a hypoxic-ischemic encephalopathy model (HIE) in neonatal rats, and to study the brain protection of recombinant human erythropoietin (EPO) and the changes in the intestinal flora diversity of neonatal rats HIE. Do you provide experimental evidence for the clinical application of EPO in the treatment of neonatal hypoxic-ischemic encephalopathy?

　　Method: Choose 7-day-old SD rats to create HIE models and randomly divide them into HIE model groups? EPO experimental group? In the control group, how does immunohistochemistry observe the expression changes of nestin, and at the same time collect the feces of each group of rats, and observe the changes of intestinal flora through 16sRNA sequence?

　　Result: The nestin expression level of rats in each group was significantly different at the same time point (P\u003c0.05), the control group was the lowest, the EPO experimental group was the highest, and the HIE model group was the second. The Shane Wiener index of the HIE model group was lower than that of the control group.

　　Conclusion: Can exogenously controlled EPO change the diversity of the intestinal flora of rats, and at the same time promote neuron growth and show protection in the HIE neonatal rat model?