动物实验 z-bo 2020-09-05 377

　　OBJECTIVE: To establish a traditional Chinese medicine Golden Thinking model of Dongpol base memory impairment, and observe the effect of Golden Thinking on the behavior and cholinergic system of mice with memory impairment caused by Dongpol base. Explore the neuroprotection of drugs.

　　Mechanism: ICR mice were randomly divided into normal control group, model group, 0.5% CMC solvent, positive control group, donepezil 0.92mg/(kg?d); Svidia medium? Low-dose group, 20? 10? 5 mg / (kg? d)? Are 18 mice in each group given 0.1 mL/10 g mouse body weight for 30 consecutive days? After the last administration, prepare the model, inject saline intraperitoneally into the control group, dissolve Eastpol base in 3mg/(kg·d), dissolve 0.9% saline in another group, and add 0.1mL/ 10g mice. The Morris water maze experiment was performed by weight injection. After the experiment, the cortex and hippocampus were collected, and the acetylcholine (Ach) content of the cortex and hippocampus was measured. What are the activities of esterase (AchE) and choline acetyltransferase (ChAT)?

　　Result: Golden Thinking can shorten the swimming distance and swimming time of the model mouse, and increase the residence time of the target quadrant; Can Golden Thinking increase the brain Ach content of the model mouse? Decrease AchE activity and increase ChAT activity?

　　Conclusion: Jin Shiwei can improve the learning and memory ability of mice with memory impairment caused by Dongpol alkali. Is this mechanism related to the cholinergic system?