动物造模,感染肺炎支原体 z-bo 2023-01-12 514

　　Objective: What is the TNF-α in the serum of mice infected with Mycoplasma pneumoniae (MP)? INF-γ content and MPN372 in lung tissue? P1? The molecular mechanism of MP infection, study the effect of Qingzao Jiufei Decoction and its degradation products on the expression of AQP5 and determine its resistance?

　　Method: 144 SPF BABL/c mice were randomly selected from the normal group (group A)? Model group (group B)? QJD group (C group)? QJD disintegrant group I (group D), QJD disintegrant group II (group E) and azithromycin group (group F), 24 mice in each group? Except for group A, the other 5 groups of mice received MP infection model treatment, and were given corresponding drug treatment after modeling. Gavage was given on the 3rd, 7th and 10th days after the model was created. Completed and after 14 days. Observe pathological sections of mouse lung tissue under a microscope, score the degree of pathological inflammation of mouse lung tissue, and calculate mouse lung index and dryness. Comparison; MPN372 using qPCR method? Determine the P1 gene content; determine the change of serum TNF-α by enzyme-linked immunosorbent assay (ELISA)? INF-γ levels in each group of mice; Western blotting to detect AQP5 protein expression?

　　Results: After MP infection, the alveolar septum thickened, the bronchiolar wall thickened, a large number of inflammatory cells were infiltrated, the lung index increased, and the dry-wet ratio decreased. TNF-α? The content of INF-γ showed an upward trend and reached the peak on the 7th day; the protein expression of AQP5 showed a downward trend and gradually increased on the 14th day; compared with the B group, from the 7th day, the C group was MPN372? Is it possible to down-regulate and up-regulate the expression levels of P1 and TNF-α, INF-γ? AQP5 expression level; the main effect of group D is MPN372? P1? It down-regulates the expression of TNF-α, the effect of group E is mainly INF-γ? AQP5?

　　Conclusion: QJD can regulate lung inflammation after MP infection, and reduce the production of MP toxin MPN372 and the expression of P1 adhesion protein. Will you up-regulate INF-γ expression? It down-regulates AQP5 protein expression and TNF-α expression. Is it one of its mechanisms of action?