动物造模,血栓通胶囊 z-bo 2022-02-04 354

　　Objective: How to study the protective effect of Panax Notoginseng Saponins (PNS) and its monomer Rg1? b1 affects the tight junction damage of vascular endothelium caused by oxygen glucose deprivation (OGD).

　　Method: Inoculate HUVEC cells in the Transwell cell compartment, and after the cells are fused, establish an experimental group: normal control group? Model group? PNS10, 20, 40 mg/L group and Rb113.52 mg/L, Rg112.44 mg/L group at the same time, under hypoxia, hypoxia and hypoglycemia for 6 hours and reoxygenation for 24 hours after drug treatment was detected after fluorescence HUVEC endothelial cell resistance and endothelial permeability changes; using laser confocal microscope and immunofluorescence to detect HUVEC cells ZO-1?. Changes in claudin-5 protein expression.

　　Result: After 6 hours of hypoxia and 24 hours of reoxygenation, the tight junction resistance between HUVEC cells and endothelial cells was significantly reduced. Compared with the normal control group, the cell permeability was significantly improved. It has been added (P\u003c0.05). In the model group, PNS20 and 40 mg/L groups can significantly inhibit the decrease of tight junction resistance and the increase of HUVEC cell permeability (P\u003c0.05). Ginsenoside Rb1 can significantly increase endothelial tight junction resistance and reduce HUVEC cell permeability (P\u003c0.05) after 24 hours of OGD6h reoxygenation. Immunofluorescence test results showed that the ZO-1 and claudin-5 proteins of HUVEC cells in the normal control group were mainly distributed around the cell membrane and arranged continuously, which is a typical feature of endothelial cells. Shows typical paving stone layout. After 6 hours of hypoxia and 24 hours of reoxygenation, the tight junction protein at the cell edge of the model group was significantly reduced, the loop structure was destroyed or disappeared, and the tight junction protein in the nucleus tended to increase. It is PNS20, 40 mg/L and Rb113.52 mg/L. Local restoration of tight junctions between cells can be observed, and it shows a cobblestone-like structure.

　　Conclusion: PNS can effectively protect the tight junction damage between vascular endothelial cells caused by ischemia.