动物造模,A型肉毒素重链 z-bo 2022-02-04 391

　　OBJECTIVE: To investigate the effect of artificially recombined botulinum toxin type A heavy chain (BoNT/A heavy chain) on the expression of local growth-related proteins in rat spinal cord injury and promote neurite regeneration. It provides a way to clarify the mechanism of A heavy chain evidence.

　　Methods: A rat unilateral lumbar spine transection injury model was established, and BoNT/A heavy chain was administered locally and intrathecally; after treatment, two-dimensional electrophoresis was performed on the local bone marrow tissue at various time points after treatment. Under the influence of BoNT/A heavy chain, execution and detection Global protein expression; GAP-43) (it selects growth-related protein 43 and upper cervical ganglion protein 10 (SCG10)) for expression and distribution. Observe by western blotting and immunofluorescence detection.

　　Results: (1) Unilateral lumbar spinal cord injury model animals showed obvious unilateral motor and sensory dysfunction; (2) BoNT/A heavy chain administration after spinal cord injury affected the local protein expression profile of the injury may give: BoNT The /A heavy chain can reverse the damage to local protein spots or based on changes, leading to further expression, especially when the MW is 35–45 kDa–18–25 kDa, it may increase or decrease. Protein spots with an isoelectric point in the range of 5-7 are obvious. (3) Western blot and immunofluorescence staining results show that BoNT/A heavy chain can promote the expression of p-GAP43 and SCG10 (P\u003c0.05). SCG10 is mainly positive for cells surrounding the injury, whether it is cytoplasm or protrusions.

　　Conclusion: BoNT/A heavy chain administration after spinal cord injury can promote the expression of selective growth-related proteins p-GAP43 and SCG10 after spinal cord injury.