动物造模,维生素D,糖尿病 z-bo 2023-01-12 505

　　Objective: To investigate the effect of vitamin D (VD) deficiency on the etiology of spontaneous diabetic rats.

　　Method: 5-6 weeks old male Zucker control mice and model mice (ZDF) were randomly divided into four groups according to their body weight, namely the normal control group (ZL)? ZDF) and VD have no model group (ZDF + VD.Def). The rats in each group were raised until 12 weeks of age. During the feeding process, monitor body weight, food intake, water consumption, urine output, urine glucose and blood sugar levels, and perform an oral glucose tolerance test at 11 weeks of age. Using HE staining, the morphological and structural changes of rat pancreatic islets were observed.

　　Results: The body weight of rats in the VD deficiency model group was significantly higher than that of the model group; the increase in water and urine output was earlier than the model group; the blood glucose level of the rats in the VD deficiency model group was significant at all stages Significantly faster, and significantly higher than the model group, about 2.0 times higher than the 12-week-old model group. The glucose tolerance of the model group was impaired. The damage to islet cells is more serious.

　　Conclusion: VD deficiency can accelerate the onset of diabetes in ZDF rats and aggravate its onset. VD deficiency is the main factor in the development of obese T2DM.