动物造模,造血干细胞 z-bo 2023-01-12 516

　　Objective: To explore the effects of different administration methods on the function of hematopoietic stem cells in mice.

　　Methods: C57 mice were injected with busulfan intraperitoneally, divided into high-dose group (40mg/kg) and low-dose group (20mg/kg), low-dose group once, high-dose group for 2 days, 20mg/kg/day . At 15 days and 30 days after administration, the proportions of peripheral blood and bone marrow cells, hematopoietic stem cells, hematopoietic progenitor cells and long-term hematopoietic progenitor cells were measured, respectively, and the proliferation ability of mouse hematopoietic progenitor cells was evaluated by CFU-GM experiment, and flow Cytometry. Long-term hematopoietic stem cells are classified by category. In vitro culture, test the function of hematopoietic stem cells through single cell colony experiment.

　　Results: Reduced the number of white blood cells and platelets in the peripheral blood of C57 mice, and reduced the proportion of hematopoietic stem progenitor cells and long-term hematopoietic stem cells, CFU-GM and single cells in the high-dose and low-dose groups of busulfan. You can reduce the number of colonies form. ability. The injury effect of the high-dose group was worse than that of the low-dose group, and there was no significant difference between the weight of the mice and the control group.

　　Conclusion: High-dose busulfan can induce hematopoietic stem cell damage 15 days after administration, providing a model and basis for studying the damage mechanism and damage protection of hematopoietic stem cells.