动物造模,富血小板血浆 z-bo 2020-09-07 372

　　Objective: To explore the effects of core decompression combined with platelet-rich plasma (PRP) on hormone-induced femoral head necrosis and tissue inhibitor of metalloproteinases/tissue inhibitor of metalloproteinases (MMP/TIMP).

　　Method: Randomly divide 42 experimental rabbits into a simple decompression group? There are 14 rabbits in each combination and control group. A bilateral steroid hormone-induced femoral head necrosis model of the simple decompression group and the combined group was established. Prepare and simulate the PRP of the combination group: 2 intravenous injections of 10μg/kg E. coli endotoxin (lipopolysaccharide, LPS), 24 hours between the two injections, the second LPS injection, prednisolone acetate 20 for gluteus maximus mg/kg, 3 injections, each injection interval is 24 hours, but the control group only injected the same amount of saline as prednisolone acetate into the gluteal muscle of model rabbits during the same period? SANFH animal model repair method: the decompression group only received core decompression, the combination group received core decompression combined with PRP implantation, and the control group was not treated? Decompression group? The combined group will take X-rays of both hips at 2 weeks, 6 weeks and 10 weeks after surgery to calculate the lane Sandu X-ray score and the percentage of new bone area. After the 10th week of shooting, the venous blood of the 3 groups of model rabbits was collected, and the 3 groups of model rabbits were killed at the same time, the bilateral femoral heads were taken out, and the MMP-2 and MMP of the right femoral skull tissue were collected. Test -9, TIMP-1 and TIMP-2, and observe the pathological changes of the mRNA expression level in the left femoral head consistently.

　　Result: MMP in postoperative decompression group? 2 and MMP? 9 is significantly higher than the combination and control group, but TIMP? 1 and TIMP? 2 Lower than the combination treatment group and the control group, but the difference is statistically significant (P\u003c 0.05); the IL-6 and bone porosity of the decompression group are also significantly higher than the combination treatment group and the control group; the above-mentioned combination group The two indicators were also higher than those of the control group (P\u003c0.05); from the perspective of X-ray scores, the combined group was higher than the decompression group at 6 and 10 weeks after surgery, and the difference was statistically significant (P \u003c0.05). On the other hand, at 2, 6, and 10 weeks after surgery, the percentage of new bone area was higher than in the combined group. In the pressure group, the difference was significant (P\u003c0.05). In the decompression group, HE staining of skull tissue sections showed a large number of discontinuous bone fragments and bone marrow necrosis, as well as signs of trabecular bone fracture. The combination group had only mild inflammatory cell infiltration, but mature trabecular bone and bone marrow tissue were already visible.

　　Conclusion: Core decompression combined with PRP can have a positive effect on the MMP/TIMP of rabbits in the SANFH model, and effectively promote tissue repair. This mechanism deserves more detailed study.