Ⅰ型干扰素,小白鼠,裸鼠 z-bo 2020-07-17 704

Experimental study on in vitro fertilization of mice with type Ⅰ interferon receptor deletion

　　Methods Two types of I type interferon receptor-deficient mice (IFN-α R- /-, IFN-α/ β R- /-) and background wild-type mice (C57BL/ 6) were subjected to in vitro fertilization and embryo transfer, respectively. In each group, 5 mice were over-excluded, repeated 3 times, and the relevant data was recorded to analyze whether the lack of interferon receptors affected the in vitro fertilization of mice. Two types of I-type interferon receptor-deficient mouse gametes and C57BL/ 6 mouse gametes were subjected to in vitro fertilization hybridization experiments, each group of 5 mice, 3 replicates, to explore the type I interferon receptor deletion on male and female gametes in vitro The impact of fertilization. At the same time, optimize the conditions of in vitro fertilization and explore techniques to increase fertilization rate. Five mice per group were repeated three times.

　　 Results The average in vitro fertilization rate of two Type I interferon receptor-deficient mice was lower than that of background strain C57BL/6 mice, and the difference between the groups was significant (P<0.05). The in vitro fertilization rate of interferon α receptor-deficient mice was higher than that of interferon α and β receptor double-deletion mice, and the difference between the groups was significant (P<0.05). The sperm of the two type I interferon receptor-deficient mice and the C57BL/ 6 mouse egg cell fertilization rate in vitro are higher than the in vitro fertilization rate of their egg cell and C57BL/ 6 mouse sperm, the difference between the groups is significant (P<0 . 05). By extending the sperm capacitation time to 1 h or adding 1 mmol / L reduced glutathione (GSH) to the capacitation fluid and fertilization fluid, the in vitro fertilization rate can be improved in vitro, and the difference between the corresponding groups is significant (P< 0 . 05).

Conclusion The deletion of type I interferon receptor may lead to a decrease in the in vitro fertilization rate of the corresponding strain of mice, and the effect on egg cells is more significant than that of sperm. By appropriately prolonging the sperm capacitation time or changing the capacitation and fertilization components, it can be improved In vitro fertilization rate.