动物造模,高频电磁辐射 z-bo 2020-09-07 402

　　OBJECTIVE: To explore the establishment of an injury model of high-frequency electromagnetic radiation on the emotional and cognitive behavior of mice, and to provide experimental basis for studying the mechanism and protection of radiation injury.

　　Method: The high-frequency electromagnetic reverberation chamber can produce continuous and uniform electromagnetic radiation waves. We exposed the mice to 1d, 3d and 7d high-frequency electromagnetic radiation boxes, and performed a series of behavioral tests to observe the autonomic nervous activity, anxiety-like behavior, depression-like behavior and cognitive function of the mice in the radiation group. The change.

　　Results: Exposure to high-frequency electromagnetic radiation (frequency 3GHz, SAR value 4W/kg) for 4 consecutive days a day, continuous exposure for 7 days will not affect the autonomic nervous activity of the mice, but the mice in the radiation group have been opened can significantly increase The number of times (P\u003c0.05) and time (P\u003c0.05) of entering the central area of the site; Compared with the unexposed group, the mice in the radiotherapy group showed the number of times in the elevated maze experiment (P\u003c0.05) And the time to enter the open arm was significantly reduced (P\u003c0.01); at the same time, through forced swimming and tail suspension experiments (P radiation dose fixed time in mice was also significantly increased. Compared with the unexposed group , This group of mice significantly reduced the recognition index of new objects in the new object recognition experiment (P\u003c0.01).

　　Conclusion: Continuous high-frequency electromagnetic radiation (frequency 3GHz, SAR value 4W/kg) can cause anxiety-like or depression-like behaviors and cognitive impairment in mice in a time-dependent manner, and is accompanied by preventive and preventive measures. Provide experimental research based on treatment technology.