动物造模 z-bo 2022-04-26 409

　　Objective: To explore a fast, convenient, and efficient way to extract dorsal root ganglia (DRG) in rats—to search for and extract DRG against the direction of nerve walking.

　　Method: The traditional method and the new method of opening the intervertebral foramen were used to extract DRG, and the time consumed by the two methods of extraction and the number of complete DRGs were compared.

　　Results: Using traditional methods, the number of complete dorsal root ganglia from L3 to 5 segments per rat was (3.08 ± 1.31), and each DRG took (5.58 ± 1.21) minutes on average. The number of complete dorsal root ganglia from L3 to 5 segments extracted by the new method per rat is (4.29 ± 1.08), and each DRG takes (1.69 ± 0.91) minutes on average. Compared with the traditional method, the difference is significant (P<0.05).

　　Conclusion: The new method of searching and extracting DRG against the direction of nerve walking can more efficiently extract the complete rat dorsal root ganglion, which provides a more reliable method for the culture and morphological study of dorsal root ganglion cells in the later stage of the experiment Organize materials.